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In addition, callus production (callogenesis) from explants in tissue culture can potentially be used to increase plant numbers through organogenesis ( Page et al., 2021). Hence, there is interest in using tissue culture methods to propagate cannabis, as it is recognized as a means to potentially increase plant numbers of desired genotypes (micropropagation), and maintain them in a controlled and stable environment (preservation) ( Monthony et al., 2021). However, maintaining donor plants to be used as a source of vegetative cuttings can be time-consuming and space-intensive. However, vegetative cuttings can lose vigor, since donor (mother or stock) plants can be affected by fungal pathogens and viruses that can reduce their growth and quality ( Punja et al., 2019 Punja, 2021). sativa is allogamous ( Punja and Holmes, 2020). Vegetative cuttings are the conventional method for commercial propagation of cannabis to ensure rapid propagation of desired genotypes without the introduction of genetic variability resulting from sexual reproduction as C.
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sativa.Ĭannabis sativa L., a member of the Cannabaceae family, is a dioecious, annual flowering plant that has been cultivated for thousands of years for its fiber (as hemp) and medicinal and psychotropic properties (as cannabis or marijuana). The procedures described here have potential applications for research and commercial utility to obtain plantlets in stage 1 tissue cultures of C. The success in recovery of plantlets from meristems and nodal explants is influenced by cannabis genotype, degree of endophytic contamination of the explants, and frequency of rooting. The callogenesis response of leaf explants of 11 genotypes on MS medium without activated charcoal was 35% to 100%, depending on the genotype organogenesis was not observed.
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sativa and has potential for obtaining pathogen-free plants. The development of plantlets from meristems is described for the first time in C. Following acclimatization, plantlet survival in hydroponic culture, peat plugs, and rockwool substrate was 57, 76, and 83%, respectively. Sodium metasilicate improved the visual appearance of the foliage and improved the rate of rooting. To obtain rooted plantlets, shoots from meristems and nodal explants of genotype Moby Dick were evaluated for rooting, following the addition of sodium metasilicate, silver nitrate, indole-3-butyric acid (IBA), kinetin, or 2,4-D.
VISUAL LIGHTING LC 075 M S AL DRIVER
The effect of Driver and Kuniyuki Walnut (DKW) or MS basal salts in media on shoot length and leaf numbers from nodal explants was compared and showed genotype dependency with regard to the growth response.
VISUAL LIGHTING LC 075 M S AL PLUS
Seven commercially grown tetrahydrocannabinol (THC)-containing cannabis genotypes (strains) showed significant differences in response to shoot growth from meristems and nodal explants on Murashige and Skoog (MS) medium containing thidiazuron (1 μM) and naphthaleneacetic acid (0.5 μM) plus 1% activated charcoal. Various sterilization methods were tested to ensure shoot development from nodal explants by limiting the frequency of contaminating endophytes, which otherwise caused the death of explants. In this research, we evaluated variables that can influence the success of shoot growth and plantlet production in tissue cultures of drug-type Cannabis sativa L. Tissue culture approaches are widely used in crop plants for the purposes of micropropagation, regeneration of plants through organogenesis, obtaining pathogen-free plantlets from meristem culture, and developing genetically modified plants. Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada.Holmes, Samantha Lung, Danielle Collyer and Zamir K.